# Quick check of run quality Checking coverage on the target regions can be a quick way to determine if the map/align step ran as expected. Following the enrichment and sequencing guidelines, the Mean Target Coverage (MTC) Depth for tumor samples is expected to be ≥130x, for normal samples ≥50x. This metric is called "Average alignment coverage over target region" and can be found in the \.target\_bed\_coverage\_metrics\_\.csv file. Note that results for "Target bed" should be evaluated, as DRAGEN Report defaults to genome, while this is a target-based, WES, assay. Another metric to check is "Aligned reads in target region", which gives a percentage of the reads in the target region relative to the entire genome and can be thought as Percent Read Enrichment.\ \ Other quality metrics to check can include the percentage of Mapped Reads (aim of ≥ 90%) and the Insert length (aim of ≥ 125bp) in the \.mapping\_metrics.csv file. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.ffpe-exomev2p5.illumina.com/additional-information/quick-check-of-run-quality.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.